Top Secret Nutrition: N.O. 370

N.O. 370™ is a new type of performance enhancing supplement that helps maximize your workouts and recovery by supporting increased blood flow and muscle energy.* N.O.370™ is the only performance enhancing supplement on the market that combines the power of Astravar™ and ActiGin™ to achieve these goals.*

Adrenalin may be able to help you do one more repetition, however, increasing blood flow and muscle energy may also help you:

Blood flow and muscle energy are the two critical factors to maximize fitness. N.O.370™ was developed to support both blood flow and muscle energy, making it not only unique, but also extremely effective.* If you want to improve the results of your workouts, taking N.O.370™ before and after you exercise may provide just the boost you are looking for!*

N.O. 370™ has several unique and effective attributes that make it effective in promoting blood flow and muscle energy, but the primary two factors are:

Several studies address the benefits of the vasodilating effects of nitric oxide.¹ The main goal of a performance enhancing supplement is to deliver the nitric oxide to your muscle cells, where it will be most effective.

We designed N.O. 370™ to support and enhance nitric oxide production via the L-arginine nitric oxide pathway.*

Many cells in the body produce nitric oxide (NO). However, its production by vascular endothelial cells is particularly important in the regulation of blood flow. Within the endothelial cell, NO is produced from the amino acid L-arginine by the enzymatic action of nitric oxide synthase (NOS). Blood plasma levels of L-arginine and L-citrulline are key components to the production of nitric oxide. For L-citrulline to work it must first be converted to L-arginine and then L-arginine must pass from the blood stream into the endothelial cells in order for nitric oxide to be produced.

Top Secret Nutrition developed N.O.370™ with innovative ingredients to tap into this process to support nitric oxide production.*

1.- Astravar™ - is a natural, plant-derived compound that is extracted from highly fractionated Panax notoginseng and Astragalus membranaceus. Research suggests that Astravar may improve the absorption of various nutrients at the cellular level.* This means that a greater amount of these nutrients are transported from the blood stream into cells, rather than just floating in the blood stream, for truly improved bioavailability.²*

Astravar may help enable up to 62% more L-arginine to become bioavailable so it can be converted to nitric oxide.* Research suggests that the ingredients in Astravar can increase eNOS (endothelial nitric oxide synthase), the enzyme that prompts nitric acid production in endothelial cells.³*

2.- L-Arginine - The amino acid L-Arginine is a pre-cursor to nitric oxide production. Research suggests that plasma levels of L-arginine are a key determinant in endothelial nitric oxide production.^4,5 However, orally supplemented L-arginine can be degraded in the small intestine by the enzyme arginase, converting it to L-ornithine and reducing its availability for nitric acid production.* While other supplement manufacturers have simply switched from L-arginine to L-citrulline, which is not metabolized during digestion,⁴ Top Secret Nutrition has developed an innovative combination of ingredients to further increase the availability of L-arginine.* The combination of L-arginine and the following two ingredients help support increased nitric oxide production in your body.*

3.- L-Citrulline - The amino acid L-citrulline is a pre-cursor to L-arginine. It is also a byproduct of endothelial nitric oxide production. During the cycle, L-citrulline is converted into L-arginine, creating more blood level L-arginine to be used to create nitric oxide. Research suggests that oral supplementation of L-citrulline is not metabolized in the intestine or liver and is thus a good supplement to support increased nitric oxide levels.*⁴

4.- L-Norvaline - Blood levels of L-arginine are converted to L-ornithine by the enzyme arginase, effectively reducing the amount of L-arginine available for nitric oxide production. L-Norvaline is an arginase inhibitor, allowing more L-arginine to be available to produce nitric oxide.*⁵ The addition of L-norvaline to N.O.370™ helps your body receive more of the L-arginine in the supplement.*

5.- Vinitrox™ - A unique formulation of grape and apple extract with a high polyphenol content, ViNitrox acts synergistically with L-arginine to promote the production of endothelial NO.* Research suggests that the synergistic interaction of grape and apple extract increases nitric oxide.* One hypothesis is that the grape polyphenol extract stimulates the production of nitric oxide and the apple polyphenol extract helps prevent the degrading of nitric oxide by blocking NADPH oxidase, an enzyme complex that reduces the amount of bioavailable nitric oxide.*⁶

When you lift weights and perform explosive movements, you are using anaerobic energy. This is when you are using your fast twitch type II muscle fibers (the ones that increase in size). Anaerobic Energy consists of two sub systems 1. The Phosphagen System and 2. The Anaerobic Glycolysis System. These two systems combined can provide up to 2-3 minutes of energy. The objective of N.O.370™ is to maximize Anaerobic Energy.*

How does N.O.370™ Support Optimum Anaerobic Energy?

PHOSPHAGEN SYSTEM
The phosphagen system is used during the highest levels of exercise intensity, but intramuscular stores of phosphocreatine are very limited and can only provide energy for exercises lasting up to 30 seconds. With full creatine stores however, the phosphagen system can re-energize faster. This is why we have included creatine in N.O.370™.

CREATINE - You may already be familiar with creatine, but there is more to the story than you may know. Creatine is an acid that is produced in the liver and made from three amino acids: arginine, glycine, and methionine. It supplies energy to muscle cells and helps recycle ATP energy. When ATP energy is spent it becomes ADP, then creatine phosphate converts ADP back into ATP and cycle continues. This process has many advantages, including increased muscular contraction time that allows you to work out harder before experiencing muscle failure. The duration of energy this system provides is about 4-30 seconds before it needs to be replenished. Research suggests that creatine supplementation may enhance anaerobic ATP synthesis for fast twitch type II muscle fibers.*⁷ Studies on creatine suggests that it may increase strength, endurance, and recovery.*^8,9,10

ANAEROBIC GLYCOLYSIS SYSTEM

The anaerobic glycolysis energy system exclusively uses glucose (and glycogen) as a fuel in the absence of oxygen or, more specifically, when ATP is needed at rates that exceed those provided by aerobic metabolism. The consequence of rapid glucose breakdown is the formation of lactic acid (more appropriately, lactate at biological pH levels). This is when you "feel the burn" during exercise.

Muscle Glycogen is an often-overlooked exercise component that is the key to muscle size, strength, and endurance. If you don´t have enough muscle glycogen you cannot push your muscles to grow, since muscle glycogen is a primary energy source during high intensity exercise and it can be depleted over repeated days of heavy training. The depletion of muscle glycogen is even greater when there is not sufficient time between workouts for it to be replenished.

How long does it take to replenish glycogen stores? It depends on the amount of carbohydrates in your diet and when these are consumed. Generally, it takes 24-48 hours to fully replenish glycogen, which is quite a bit longer than the time it takes for your muscles to consume it. Research also suggests that when muscle glycogen is depleted, power output is reduced.¹¹ You do not want to get to the gym with depleted glycogen stores if your goal is to pack on muscle. Additionally, you want the ability to quickly replenish glycogen when it´s depleted during the workout. This is why we included ActiGin in N.O.370™. ActiGin™ helps enhance muscle glycogen replenishment.*

ACTIGIN™ - Top Secret Nutrition included this proprietary high-performance sports ingredient to help you exercise at a higher intensity level for a longer duration and with less recovery time.* Actigin supports this by helping to improve muscle glycogen replenishment.* The ginsenosides found in ActiGin™ have been suggested in several in vitro studies to up-regulate associated gene expression and proteins that would in theory increase the glycogen synthesis in muscles.*¹² A human study on glycogen synthesis rate was later carried out on ActiGin. The results of that study indicated that ActiGin may help restore muscle glycogen by up to an amazing 370% after exercise.*¹² What does this mean for your fitness regimen?

Studies have shown that one of the keys to muscle recovery and muscle glycogen replenishment after a high-intensity workout is taking a supplement that contains carbohydrates, creatine, and protein.*^13,14 Further research has suggested that caffeine is a beneficial addition to this mix. In a study performed in Australia, scientists presented the first evidence suggesting that caffeine co-ingested with carbohydrates after a bout of glycogen-depleting exercise had an additive effect on rates of post-exercise muscle glycogen accumulation.*¹⁵

Now, here is the secret: Add N.O.370™ to your post-workout supplement with protein and carbohydrates. The synergistic combination of clinically-studied Astravar to improve absorption, ActiGin™ to maximize muscle glycogen replenishment, caffeine, creatine, and the nitric oxide boosting ingredients to help shuttle nutrients to your recovering muscles, will put you well on your way to supporting faster recovery, improved muscle growth, and ready for your next workout.*

This product is not a high-stimulant supplement that will make you feel edgy, make your heart beat fast, or cause you to crash at an alarmingly quick rate. This product is for someone who wants an edge to achieve real results - a big edge. If you´re at the level where doing one more set, lifting heavier weight, and working out the next day at the same intensity will have an impact on your fitness level, N.O.370™ is for you.

Q. Why didn´t you include any carbohydrates in N.O.370™?
A. There are no carbohydrates in N.O.370™ for two reasons. 1.- Many athletes want to control their carbohydrate intake, and not having them in a supplement makes this easier, and 2.- If you are eating adequately your carbohydrate stores should be sufficient to replenish glycogen.

Q. I have been using a high-stimulant pre-workout supplement. What are the differences I may feel by taking
N.O.370™, which is a low-stimulant pre-workout supplement?
A. Our decision to develop a low-stimulant pre-workout supplement was driven by our objective to create a product that promoted maximum blood flow to the muscles and maximum muscle glycogen replenishment.* A high-stimulant supplement has some competing attributes to these objectives. However, if you like that high-stimulant feeling, you can stack those supplements with N.O.370™.

Q. Is there a reason you did not use Beta-alanine in N.O.370™?
A. Firstly, we like Beta-alanine. There are numerous studies on its beneficial effect on exercise. We decided not to put Beta-alanine in this version on N.O.370 for simple reasons. 1.) Some people do not like the "tingles" you get from the Beta-alanine, and 2.) Beta-alanine is easy to stack with N.O.370 for those who like the product.

Q. I have hit a plateau with my bench press and want to break my personal record, how can N.O.370™ help?
A. If you have been training and have been increasing your bench press and have hit a plateau, try the following method.

Do a full-body workout with the exception of chest, our goal is to deplete muscle glycogen stores as much as possible. Do one to two sets per body part.

Begin consuming high glycemic carbohydrates, 100 grams per meal for at least six meals. In the morning take one serving of N.O.370™ and in the early evening take another serving of N.O.370™. Do not work out; this is the loading phase.

This is record-breaking day. Because you have supercompensated muscle glycogen stores and have supported that with N.O.370™, you will be primed to break personal records in strength lifts. Additionally, you will probably also get some of the best pumps of your life.* Since every gram of glycogen is stored with approximately three grams of water, a doubling of glycogen stores due to glycogen supercompensation is likely to increase the size of muscles. Exercise up-regulates the body´s ability to store glycogen and trained athletes store somewhere around 800 grams of muscle glycogen. Supercompensation may be able to double that. Add 800 grams of glycogen plus 2400 grams of water to an athlete´s muscle tissue and you will get a 7.2-pound weight gain from a single carbohydrate depletion/carbohydrate loading cycle. Therefore a bodybuilder can potentially gain a significant amount of apparent mass with successful glycogen supercompensation.

Take an N.O.370™ prior to workout along with a high glycemic carbohydrate, and then go break records and rip the sleeves of your shirt!

(1) Tschakovsky, M., and Joyner, M. "Nitric Oxide and Muscle Blood Flow in Exercise." Applied Physiology, Nutrition & Metabolism, 33.1 (2008): 151-160.

(2) Tsuchung Chang, Wen-liang Chang, and m Shu-fun Huang. "A Clinically Validated Natural Ingredient that Transports Significantly Greater Amount of Nutrients in Blood Stream into Cells for Truly Improved Bioavailability." Nuliv Science.

(3) "Panax notoginseng and its Components via Stimulation of Endothelial-dependent Vessel Dilatation." Vascul Pharmacol. 2012 Jan 3. [Epub ahead of print]

(4) "Therapeutic Use of Citrulline." Cardiovasc Drug Rev., 24.3-4 (2006): 275-90.

(5) "Arginase Inhibitor in the Correction of Endothelial." Int J Hypertens. 2011;2011:515047. Epub 2011 Jun 15.

(6) "Mechanistic Effect of ViNitrox™: Up-regulation of Endothelial Nitric Oxide Synthesis (eNOs)." Bio serae Laboratoires s.a.

(7) "Creatine Supplementation Enhances Anaerobic ATP Synthesis During a Single 10 sec Maximal Handgrip Exercise." Mol Cell Biochem. 244.1-2 (2003): 105-12.

(8) "Effect of Creatine Supplementation on Aerobic Performance and Anaerobic Capacity in Elite Rowers in the Course of Endurance Training." Int J Sport Nutr Exerc Metab. 13.2 (2003): 173-83.

(9) "Effects of Creatine on Isometric Bench-press Performance in Resistance-trained Humans." Med Sci Sports Exerc. 34.7 (2002): 1176-83.

(10) "Creatine Supplementation Enhances Muscle Force Recovery in Healthy Individuals." Journal of the International Society of Sports Nutrition, 6:13 (2009).

(11) "Effect of Glycogen Depletion on the Ventilatory Response to Exercise." J Appl Physiol. 54.2 (1983): 470-4.

(12) "ActiGinTM on Glycogen Synthesis in Human Muscles." Internal publication NuLiv Science International BioMolecular Research Institute

(13) Effects of supplement timing and resistance exercise on skeletal muscle hypertrophy. Med Sci Sports Exerc. 2006 Nov;38(11):1918-25.

(14) Postexercise muscle glycogen recovery enhanced with a carbohydrate-protein supplement. Med Sci Sports Exerc. 2006 Jun;38(6):1106-13.

(15) High rates of muscle glycogen resynthesis after exhaustive exercise when carbohydrate is coingested with caffeine. J Appl Physiol. 2008 Jul;105(1):7-13. Epub 2008 May 8.